Figure 3: Optimization of LAMP reaction temperature conditions. | Scientific Reports

Figure 3: Optimization of LAMP reaction temperature conditions.

From: Establishment and application of a novel isothermal amplification assay for rapid detection of chloroquine resistance (K76T) in Plasmodium falciparum

Figure 3

(A) Gel electrophoresis of LAMP product to test the temperature range: M- 100 bp ladder, lane 1 to lane 9 shows the tested temperature range 57–65 °C the best amplification (ladder like pattern) was observed at 61 °C indicated in lane 5. (B) Assessment of temperature range by calcein color change visualization: tube no. 1 to 9 indicate the tested temperature range 57–65 °C, there was no color change at 64 °C and 65 °C indicated in tube no. 8 and 9 because the reaction was not amplified at this range and it was reconfirmed on 3% agarose gel as shown in lane 8 and 9.

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