Table 1 Senisitivity, specificity and accuracy of LAMP and PCR-RFLP versus gold standard sequencing.

From: Establishment and application of a novel isothermal amplification assay for rapid detection of chloroquine resistance (K76T) in Plasmodium falciparum

 

Measure of agreement with gold standard sequencing (κ)

Sensitivity

Specificity

Accuracy

LAMP- gel

1.00

100%

100%

100%

LAMP- visible

1.00

100%

100%

100%

PCR-RFLP

0.92

97%

94%

96%

  1. Kappa coefficient (κ) statistics range (1 to −1), p < 0.0005 measures the observed percentage of agreement between tests. LAMP-gel, Loop mediated isothermal amplification by gel electrophoresis; LAMP-visible, Loop mediated isothermal amplification visualized by naked eye; RFLP, Restriction fragment length polymorphism.
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