Figure 1: Temporal and spatial uptake of YO-PRO-1 as a function of pulse number and frequency. | Scientific Reports

Figure 1: Temporal and spatial uptake of YO-PRO-1 as a function of pulse number and frequency.

From: Calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields in U87 human glioblastoma cells

Figure 1

(a) Change in fluorescence over time from live cell imaging experiments on YO-PRO-1 uptake into U87 cells following application of 10 ns pulses at varying pulse numbers and repetition rate frequencies. The start of pulse application is represented by a green arrow. Error bars show S.E. 0P n = 5, 1P n = 4, 10P 1 Hz n = 4, 10P 10 Hz n = 7, 10P 100 Hz n = 4, 100P 1 Hz n = 7, 100P 10 Hz n = 4, 100P 100 Hz n = 4. (b) Representative live cell images of YO-PRO-1 cellular uptake following 100, 10 ns pulses at 10 Hz. The pulse train was applied just after the image capture at 27 seconds. The images are pseudocoloured for contrast and the colour calibration bar represents arbitrary units of fluorescence. The electrode orientation is marked by the + and − in the first images of the series.

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