Figure 3

Effects of exposure to polystyrene microbeads of different diameters (0.05, 0.5, and 6 μm) on ROS production and phosphorylation of MAPK signaling proteins: (A) ROS levels, (B) ROS levels with NAC treatment (0.5 mM), (C) phosphorylation of MAPK proteins, and (D) phosphorylation of MAPK proteins with NAC treatment (0.5 mM). All polystyrene microbeads were administerd at 20 μg/ml. Levels of ROS are represented as % of controls. Different letters above columns indicate significant differences, defined as P < 0.05.