Figure 1: 90Sr-induced double-strand breaks and DNA repair. | Scientific Reports

Figure 1: 90Sr-induced double-strand breaks and DNA repair.

From: DNA damage induced by Strontium-90 exposure at low concentrations in mesenchymal stromal cells: the functional consequences

Figure 1

(a) Quantification of γ-H2AX foci per nucleus in rat BMSCs after 30 minutes, 24 and 72 hours of exposure to 90Sr or external 1 Gy γ-irradiation used as a positive control (Student’s t-test, *p < 0.05 and **p < 0.001, n = 3). (b) Representative immunostaining of γ-H2AX in MS5 control cells and after 30 minutes (upper panel) or 7 days (lower panel) of exposure to 10 kBq.ml−1 of 90Sr. γ-H2AX foci are stained in green and are indicated by the arrows, nuclei are stained in blue. (c) Quantification of γ-H2AX foci per nucleus in MS5 cells after 30 minutes, 24 hours, 72 hours and 7 days of exposure to 90Sr or external 1 Gy γ-irradiation as positive control (ANOVA on Ranks, H = 372.996, dof = 3, **p < 0.001). (d) Representative images of Western blot for Mre11, Ku70 and Rad51 proteins, from 1 hour to 7 days of exposure. GAPDH represents the loading control. Abbreviations: 0: Control cells; 1: 90Sr 1 kBq.mL−1; 10: 90Sr 10 kBq.mL−1. Full length blots uncorrected for contrast are presented in Supplementary Figure 1.

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