Figure 4: NLS arrested cell cycle progression at the G1/S phase.

(A) PC3 and DU145 cells were treated with 60 and 120 μg/ml NLS for 24, 48, and 72 h, and the cell cycle distribution was evaluated after propidium iodide (PI) staining. Data are representative of three independent experiments. (B) Bar plot of the cell cycle distribution of PC3 and DU145 cells. (C) Expression of cell-cycle-related proteins in PC3 and DU145 cells was determined by western blotting.