Figure 1: Basic principle of operation of the pipette integrated microfluidic cell isolation technology.
From: Microfluidic cell isolation technology for drug testing of single tumor cells and their clusters
(a) Schematic showing preparation of cartridge by sequential aspiration of oil and cell-laden sample, followed by a single step dispensing into the microfluidic device that has been prefilled with oil. The microfluidic device contains 60 parking loops. Each loop has a bypass channel and a lower branch containing a trap that can park a droplet of volume 30 nL. (b) Time-stamped images of plug motion through the device. The sample plug (blue dye solution) fills the channels and traps and is sequentially digitized by the oil plug producing an array of static droplets of uniform size.
