Figure 3: Viability of tumor cells in drop environment.
From: Microfluidic cell isolation technology for drug testing of single tumor cells and their clusters
(a) Comparison of cell viability of MCF-7 breast tumor cells and CCRF-CEM leukemia cells in the liquid drop environment, measured over 24 hrs. For all time points, the difference in viability between the two cell lines is not statistically significant (p > 0.05: ns (not significant)) suggesting that the level of anoikis is not very different between the two cell lines. The error bars represent standard deviation from n = 12 trials. (b) Cell viability of MCF-7 cells in drops accessed over 7 days without any media change. Up to 3 days, more than 90% of cells survived. From day 4, the viability of cells decreased. Cell viability analysis was based on 1287 cells, with each drop containing ~10 cells. (c) Changes in drop volume stored in microfluidic device over 7 days. The drop volume (V) compared to the initial drop volume (V0) is less than 11% over 7 days. The average volume ratios (V/V0) and standard deviations were calculated from 3 replicates.
