Table 1 Selected up-regulated genes of macrophages phagocytizing iRBC as determined by DNA microarray analysis and qRT-PCR.

From: Involvement of β-defensin 130 (DEFB130) in the macrophage microbicidal mechanisms for killing Plasmodium falciparum

Gene

Genbank Accession number

Fold changea a

Microarray

qRT-PCR b

qRT-PCR c

SMTNL2

NM_198501

4.613

7.20 ± 8.59

14.57 ± 11.72

DEFB130

NM_001037804

4.569

4.49 ± 3.93

3.45 ± 1.75

RIT2

NM_002930

4.543

5.74 ± 2.53

4.11 ± 6.60

SYTL4

NM_080737

4.459

5.87 ± 3.77

6.20 ± 4.60

PRSS41

NM_001135086

3.912

1.71 ± 0.85

3.44 ± 3.06

GRP

NM_002091

2.726

4.62 ± 4.95

3.38 ± 1.80

ADAM19

NM_033274

2.209

1.54 ± 1.30

1.72 ± 0.73

  1. aFold change indicates the mean expression level of the gene in macrophages co-cultured with iRBCs normalized to that in macrophages co-cultured with RBCs.
  2. bResults of qRT-PCR are from the same samples used for the microarray. Results represent the mean fold change ± SEM of three independent experiments.
  3. cResults of qRT-PCR are from pooled data of samples collected from three individual donors. Results represent the mean fold change ± SEM (n = 3).
Back to article page