Figure 5: MMF induces alterations in the 5′ UTR of OSGIN1 in human spinal cord astrocytes. | Scientific Reports

Figure 5: MMF induces alterations in the 5′ UTR of OSGIN1 in human spinal cord astrocytes.

From: The NRF2 transcriptional target, OSGIN1, contributes to monomethyl fumarate-mediated cytoprotection in human astrocytes

Figure 5

(a) Agarose gel electrophoresis of 3′ and 5′ RACE products of OSGIN1 following 0, 10, 30, or 60 μM MMF in astrocytes. (b) Quantification of a using densitometry. Data graphed as relative density compared to 0 μM MMF and normalized to 1 (dashed line). 3′ and 5′ RACE data are normalized to their respective 0 μM MMF values. (c) DNA sequencing results of 3′ and 5′ RACE products from a identifying two alternate variants of OSGIN1, V1 and V2. Underlined letters indicate potential start (ATG) and stop (TGA) codons identified in the V2 variant following nucleotide substitutions indicated in red. (d) qRT-PCR of OSGIN1 transcript variants identified in c (V1 and V2) following treatment of human astrocytes with 0, 10, or 30 μM MMF. Data graphed as relative expression of gene modulation relative to individual variants (V1 or V2) treated with 0 μM MMF and normalized to 1 (dashed line). Mean ± SD shown for n = 2/condition. ##p < 0.01 and ####p < 0.0001 (two-way ANOVA with Tukey’s multiple comparisons) compared with 0 μM MMF. *p < 0.01 and ****p < 0.0001 (two-way ANOVA with Tukey’s multiple comparisons) comparing V1 vs V2 at either 10 or 30 μM MMF.

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