Figure 6
From: Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution
2-D and 3-D LSFM detection of GFP-tagged ion-channels in a 7.5 month-old adult mouse heart (see Movie S6 ). (a–c) Cross-sectional slices on the (a) XY- (b) YZ- (c) XZ-planes reveal that the spatial distribution pattern of ROMK channels. Arrows indicate the specific fluorescently tagged ROMK channels, and gray scale encodes the optical intensity. (d) 3-D rendering of the reconstructed heart contrasts the ROMK channels. The inset shows the translucent heart after CLARITY in the tube. Scale bar: 1 mm.
