Figure 2: Overexpression of miR-125b promoted EMT in CRC cells. | Scientific Reports

Figure 2: Overexpression of miR-125b promoted EMT in CRC cells.

From: CXCL12/CXCR4 axis induced miR-125b promotes invasion and confers 5-fluorouracil resistance through enhancing autophagy in colorectal cancer

Figure 2

(A,B) HCT116 and SW620 cells were transfected with 100 nM miR-125b mimics (125 m) or inhibitors (125i) for 48 h. The levels of miR-125b were determined by RT-qPCR assay. (C,D) The cells were treated as indicated above, the mRNA levels of E-cadherin, vimentin and CXCR4 were determined by RT-qPCR assay. (E) The levels of ZEB1, Snail, vimentin and E-cadherin were determined by Western blot assay. Bar graphs indicated the relative levels of the proteins normalized to β-actin. *P < 0.05 vs. negative control. (F) HCT116 and SW620 cells were transfected with 100 nM miR-125b mimics (125 m) or inhibitor (125i) for 24 h, then were treated with or without 100 ng/μl CXCL12 for 24 h. The capacity of cell invasion was evaluated by transwell assay. *P < 0.05 vs. negative control (NC). #P < 0.05 vs. NC and 125i groups, respectively.

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