Figure 6: Staufen1 knockdown inhibits RMS tumour growth in a xenograft model. | Scientific Reports

Figure 6: Staufen1 knockdown inhibits RMS tumour growth in a xenograft model.

From: Novel Roles for Staufen1 in Embryonal and Alveolar Rhabdomyosarcoma via c-myc-dependent and -independent events

Figure 6: Staufen1 knockdown inhibits RMS tumour growth in a xenograft model.

Flank injections of 6-week-old female SCID-488 mice with ERMS (RD) or ARMS (RH30) cells following 72 hours of Control (CTL) or Staufen1-shRNA (shStau1) expression. To determine Staufen1 knockdown pre-injection, cells were collected for western blot analysis with (a) anti-Staufen1 antibodies and GAPDH as a loading control in ERMS (RD) and ARMS (RH30) cells. Quantification is represented as a percentage relative to CTL (n = 1, batch culture). (b) Representative endpoint images of ERMS (RD) and ARMS (RH30) at 72 days and 31 days post-injection, respectively. (c) Tumour weight from CTL or shStau1 at endpoint for ERMS (RD) (n = 7) and ARMS (RH30) cells (n = 6). (d) Tumour volume measurements of ERMS (RD) and ARMS (RH30) tumours over time (n = 7 and n = 6, respectively). Data are Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

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