Figure 3: Single cell force spectroscopy using MV3 cells with different NHE1 expression levels. | Scientific Reports

Figure 3: Single cell force spectroscopy using MV3 cells with different NHE1 expression levels.

From: Extracellular protonation modulates cell-cell interaction mechanics and tissue invasion in human melanoma cells

Figure 3

AFM experiments reveal that the cell-cell adhesion force, represented by the (a) maximum pulling force and the (b) adhesion energy, is lower in NHE1-overexpressing cells (0.62 nN (0.48/0.85 nN), N = 9 cells attached to the cantilever probing n = 354 cells on the underlying matrix; 1.96 fJ (0.97/2.65 fJ), N = 9, n = 315) than in MV3 control cells (1.45 nN (0.99/1.95 nN), N = 8, n = 326; 4.35 fJ (2.67/6.8 fJ), N = 8, n = 281). Statistical significance of the differences was assessed by the Mann-Whitney U test.

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