Figure 7: Functional study of DPEP1 in CRC cells.

(A) Proteins prepared from CRC cell lines LOVO, HT29, DLD1, SW480, Caco2 and HCT116, and human colon normal epithelium cell line FHC were subjected to Western blot analysis. GAPDH was used as the loading control. After transfection with DPEP1 siRNA, Control siRNA, Vector plasmid and DPEP1-expression plasmid in the cells, (B) MTT viability assay, (C) Flow cytometry and (D) Transwell assay were used to detect the cell proliferation, cell apoptosis and cell invasion ability, respectively. ***P < 0.001.