Figure 4: Cortical and trabecular femur MAR and BFR are comparable between plastic and paraffin embedded sections. | Scientific Reports

Figure 4: Cortical and trabecular femur MAR and BFR are comparable between plastic and paraffin embedded sections.

From: Quick and inexpensive paraffin-embedding method for dynamic bone formation analyses

Figure 4: Cortical and trabecular femur MAR and BFR are comparable between plastic and paraffin embedded sections.

Whole femurs were obtained from all mice and were randomly divided (left or right) between the standard protocol for plastic processing or the protocol for paraffin processing. Slides were examined at 20X magnification with conditions blinded. (a) Long bone formation rate (BFR). (b) Long bone trabecular mineral apposition rate (MAR). (c) Representative 4X magnification images of fluorochrome bands in mineralized sections of the distal femur embedded in plastic or paraffin showing the metaphyseal and diaphyseal regions. Enlarged images are taken at 20X objective power. The white scale bar is 50 μm in length. (d) Long bone cortical MAR. Values are averages ± SE. n ≥ 16 per condition. *p ≤ 0.05 by one way ANOVA.

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