Figure 2: Optimization of LAMP reaction for detection of Rhizoctonia bataticola.

(a) Optimization of temperature of LAMP. M GeneRulerTM 100 bp Plus DNA Ladder; lanes 57 °C–69 °C indicated the reaction temperatures of LAMP. (b) Optimization of time duration of LAMP. M GeneRulerTM 100 bp Plus DNA Ladder; lanes 15 m–90 m indicated the reaction time duration of LAMP. All the products were detected on the basis of 2% agarose gel electrophoresis.