Figure 4: Sensitivity of LAMP assay vs. conventional PCR for detection of Rhizoctonia bataticola using similar concentration of DNA template. | Scientific Reports

Figure 4: Sensitivity of LAMP assay vs. conventional PCR for detection of Rhizoctonia bataticola using similar concentration of DNA template.

From: Rapid and sensitive diagnoses of dry root rot pathogen of chickpea (Rhizoctonia bataticola (Taub.) Butler) using loop-mediated isothermal amplification assay

Figure 4

(a) Visual assessment of LAMP assay using SYBR Green I. (b) LAMP assay on the basis of 2% agarose gel electrophoresis. (c) Result of conventional PCR using ITS1 and ITS4 primers. M GeneRulerTM 100 bp Plus DNA Ladder; lane 101–10−7 indicated the DNA concentration in LAMP reaction starting from 10 ng (101 ng) to subsequent 10 fold diluted DNA up to 0.1 fg (10−7 ng), respectively.

Back to article page