Figure 5: Evaluation of the LAMP assay for detection of Rhizoctonia bataticola in optimized conditions (63 °C for 75 min) using DNA template from infected chickpea plants and rhizospheric soil.

M GeneRulerTM 100 bp Plus DNA Ladder; lane 1–5 Dry root rot infected chickpea plants sampled from different fields; 6 Healthy chickpea plant from field; 7–10R. bataticola inoculated chickpea plants sampled from green house; 11 healthy chickpea plant from greenhouse; 12 Rhizospheric black soil from field; 13 Rhizospheric red soil from field; 14 Sick soil from greenhouse; 15 Negative control without any DNA template; 16 Positive control with genomic DNA of R. bataticola.