Table 3 Information of the primers used for the LAMP reaction.
Primers name | Sequences (5′-3′) | Type | Primer position (nt) | Length (bp) | GC (%) | Tm |
|---|---|---|---|---|---|---|
RB_F3 | CCTCCCACCCTTTGTATACCTACC | Forward outer | 1–24 | 24 | 54.2 | 58.4 |
RB_B3 | CGCAAAGGACGGTGCCCAA | Backward outer | 336–354 | 19 | 63.2 | 61.4 |
RB_FIP (F1c+F2) | CTGCAACGTTTACTGACTGGAGTTTG-CCGATTTTGGGGGGTGGCTAGT | Forward inner | (104–129) + (65–86) | 26 + 22 | 46.2–59.1 | 58.2–61.7 |
RB_BIP (B1c + B2) | CGGATCTCTTGGTTCTGGCATCG-GAAATGACGCTCGAACAGGCATG | Backward inner | (168–190) + (291–313) | 23 + 23 | 54.2–52.2 | 60.9–58.8 |
RB_LoopF | TCCTCTGGCGGGCACTAG | Forward loop forming | 82–99 | 18 | 66.7 | 59.4 |
RB_LoopB | ACATTGCGCCCCTTGGGATT | Backward loop forming | 263–282 | 20 | 55 | 60.2 |
