Figure 3: Clone C27 contains a balanced translocation.
From: Generation of Gross Chromosomal Rearrangements by a Single Engineered DNA Double Strand Break
(A) Sequence of the wild-type (WT) chromosome 6, derivative 6 and EF1aTKHyg construct integrated on chromosome 11. Centromere and telomere are indicated; I-SceI site is boxed. Chromosome 11 sequences are in black; chromosome 6 sequences are in purple. Microhomologous nucleotides are in green. (B) Sequence of the integrated EF1aTKHyg construct, der(11), and WT chromosome 6. (C) Parental H2ax8 clone, with EF1aTKHyg integrated on chromosome 11. EF1a, TK, and HygR regions are indicated. (D) Subclone H2ax8_C27 with balanced translocation to chromosome 6 (pink). Derivative (der) 11 and 6 chromosomes and fusion points are indicated. (E–F)Chromosome 11 (green) and 6 (red) painting of H2ax8 (E) or H2ax8_C27 (F). Arrow indicates der 11 fusion. (G) Der 6 identified by hybridization of H2ax8_C27 to 456H10 (red signal) and 321F3 (green signal) BAC clones; yellow fusions are indicated with arrows.
