Figure 4: Generation of a 78 MB inversion.
From: Generation of Gross Chromosomal Rearrangements by a Single Engineered DNA Double Strand Break
(A) Nucleotide sequence of the centromeric junction (fusion of TK sequences from clone C11 to germline chromosome 11). The I-SceI site is underlined, and 2 bp of microhomology are shown in green. The centromeric germline chromosome 11 seqeunces are shown in purple; the telomeric, EF1aTKHyg sequences are in black. Centromeric (CEN) and telomeric (TEL) orientation for the germline chromosome regions are indicated. (B) Nucleotide sequence of the telomeric junction (fusion of EF1a sequences to germline chromosome 11). (C) Integration of the EF1αTkHyg vector on chromosome 11. Positions of BAC clones 402G5 and 321F3 is indicated. (D) Inversion of H2ax8_C11 caused by break at nuc. 27,022,757. (E) Hybridization of H2ax8_C11 to 402G5 (red signal) and 321F3 (green signal) Note yellow signal due to overlap of probes.
