Figure 3: Production of functional gametes and medaka individuals derived from cryopreserved whole testes.
From: Production of the medaka derived from vitrified whole testes by germ cell transplantation
(A) Embryos obtained from WT parents. (B) Embryos obtained from triploid recipients after transplantation of cryopreserved olvas-GFP TCs. (C) Sperm obtained from WT male medaka. (D) Sperm obtained from male recipients. (E,F,G) A hatching WT juvenile (top of E and F). A hatching juvenile derived from mating of recipients displaying the donor-derived phenotype of an orange body color (bottom of E) and gonads containing GFP-positive germ cells (G), suggesting that all F1 offspring were donor-derived. (H) PCR analysis, performed with tyrosinase-specific primers and gfp-specific primers, of F1 offspring derived from WT triploid recipients by cryopreserved olvas-GFP TC transplantation (F1), donor olvas-GFP medaka (D1-2) and WT recipient medaka (R1-2). (I) Efficiency of triploid and TC transplantation. Data are shown as the mean ± SEM. (J) one-month-old medaka (orange colored olvas-GFP medaka) generated from surrogate triploid WT parents by cryopreserved TC transplantation. Scale bars, 1 mm (A,E), 100 μm (G), and 10 μm (C).
