Figure 5: Production of sub-fertile mutants derived from cryopreserved TCs.
From: Production of the medaka derived from vitrified whole testes by germ cell transplantation
(A) The number of embryos derived from mating with WT black medaka, sub-fertile mutants, and surrogate triploid WT parents receiving cryopreserved wnt4 mutant TCs in a week. (B) Ovulated oocytes and embryos (arrowhead) produced by mating wnt4 mutants in a day. (C) Ovulated oocytes and embryos (arrowheads) derived from mating surrogate WT triploid recipients of cryopreserved wnt4 mutant TCs in a day. (C) PCR analysis, performed with tyrosinase-specific primers and normal wnt4b-specific primers, of F1 offspring, donor wnt4b mutants (lane D1-2), and WT recipients (lane R1-2). (D–F) WT medaka (top of D), wnt4 mutant (bottom of D), microscope sample after skeletal staining for WT (E), and that of wnt4 mutant produced by surrogate triploid WT parents (F). (G) Medaka individuals (orange colored wnt4b mutants) generated from surrogate triploid WT medaka by cryopreserved TC transplantation. Scale bars, 1 cm (D) and 1 mm (B).
