Figure 6: HeLa cell mass measurement.
(a) Different growth curves from a parent and two daughter HeLa cells measured using a 20x/0.3 NA objective over 32.6 hours. Each growth curves show the total dry mass of a single HeLa cell over time. When a parent cell divides, two new curves are generated for the daughter cells. (b) Measured averaged dry mass densities obtained from the thresholded quantitative phase imaging (tQPI) images and the hfQPI images over time. (c) Each row shows seven measurements of the dry mass density at different time points in (a). The first row contains the raw QPI images. The middle row shows tQPI images. The bottom row is for hfQPI images. (d) Scatter plot of the total dry mass of all several cells obtained from tQPI images (horizontal axis) and the hfQPI images (vertical axis) using automatic segmentation. The lines show the maximum slope, minimum slope and fitted slope using linear regression relations between these two quantities.
