Figure 3: A3B and MYBL2 upregulation lead to somatic mutation in breast cancer.
From: B-Myb Induces APOBEC3B Expression Leading to Somatic Mutation in Multiple Cancers
(A) Agarose gel electrophoresis shows 3D-PCR products amplified from foreign DNA (Luc+) at the indicated denaturation temperatures (Td). MCF7 cells were transfected with A3B, MYBL2, or empty vector together with pGL3 and pcDNA3-UGI. Total DNA was recovered for 3D-PCR analysis after transfection for 48 h. Each arrow denotes 420 bp. (B) Mutation metrics for Luc+ sequence derived from 3D-PCR amplicons at Td = 85 °C. Ten clones were directly sequenced for each sample, and the total number of bases sequenced (n) is indicated. (C) Dinucleotide context analysis for the rate of C-to-T or G-to-A mutation at the indicated sequence for the Luc+ sequence shown in (B). Asterisks indicate statistical significance compared with the expected mutation rate as assessed with the χ2 test. (D) Each data point represents the C-to-T mutation count for one sample in TCGA breast cancer dataset. Data were grouped based on low, middle, and high third ranks for A3B or MYBL2 expression. Horizontal bars indicate median values, and statistical significance between two groups was determined with the Mann–Whitney U-test.
