Table 1 The catalytic activities of wild type and mutant OleTJE.

From: Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE

Enzyme

Conversion rate (%)

Product distribution (%)

α-hydroxy myristic acid

β-hydroxy myristic acid

1-tridecene

OleTJE

76.9 ± 9.6

1.4 ± 0.1

12.5 ± 0.1

86.2 ± 2.0

R245Q

0

n.d.

n.d.

n.d.

R245H

0

n.d.

n.d.

n.d.

R245E

0

n.d.

n.d.

n.d.

R245K

0.8 ± 0.1

13.8 ± 0.1

86.3 ± 0.1

n.d.

R245A

0

n.d.

n.d.

n.d.

R245L

0

n.d.

n.d.

n.d.

C365H

0

n.d.

n.d.

n.d.

  1. Reaction conditions: wild type and mutant OleTJE (2 μM), H2O2 (220 μM), myristic acid (200 μM) in 200 μl desalting buffer were incubated at 30 °C for 16 h. All experiments were performed in duplicate. n.d.: not detected.
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