Figure 3
From: Disruption of Autolysis in Bacillus subtilis using TiO2 Nanoparticles
Fluorometery assay of DiSC3(5) stained B. subtilis cultures in 5 mM NaHCO3 in the presence of 50 ppm TiO2 (pink triangle) and absence of TiO2 (grey diamond) in the dark. Depolarization of B. subtilis membrane was monitored by measuring the fluorescence intensity at an excitation and emission wavelength of 643 nm and 666 nm, respectively, for 1 hour. Apparent equilibrium DiSC3(5) between the cell membrane and the media, as indicated by a flat-line in fluorescence, took 40 minutes.
