Figure 1: P2R antagonism modulates murine macrophage activation upon exposure to Streptococcus pneumoniae in vitro.
From: Role of purinergic signaling in experimental pneumococcal meningitis
To elucidate the role of P2R signaling in S. pneumoniae-induced macrophage activation, murine bone marrow-derived macrophages (BMDM) were exposed to D39 pneumococci (107cfu/ml) in the absence or presence of suramin or BBG. Six hours later, medium samples were withdrawn and analysed for the presence of IL-1β (a) and IL-6 (b) using commercially available assay kits. All experiments were - at least - carried out twice in triplicates. Data are given as means ± SD. *P < 0.05, compared to S. pneumoniae -challenged macrophages, #P < 0.05, compared to S. pneumoniae -challenged macrophages treated with the lower doses of the respective inhibitor, using ANOVA and Student-Newman-Keuls test for post hoc analysis.
