Figure 8: Analysis of T4 binding to plasma TTR (A) and recombinant TTR (B) by gel electrophoresis. | Scientific Reports

Figure 8: Analysis of T4 binding to plasma TTR (A) and recombinant TTR (B) by gel electrophoresis.

From: Cavity filling mutations at the thyroxine-binding site dramatically increase transthyretin stability and prevent its aggregation

Figure 8

The samples were incubated with 125I-T4 and the proteins were separated by native PAGE. Migration of TTR and other T4 binding proteins is indicated (TBG- thyroxine binding globulin; ALB- Albumin and TTR- Transthyretin). (A) Analysis of human plasma from heterozygote carriers of TTR variants TTR A108V, TTR V30M or TTR T119M and control plasma (WT) and (B) Analysis of isolated recombinant TTR variants, namely, V30M, A108, A108I, T119M and the wild type (WT) protein. Control plasma (control) was used as reference for migration. All samples were run in the same gel though not sequentially. The place where the gel was trimmed is indicated.

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