Figure 3: LOXL2 depletion diminishes UPRE and ERSE promoter activities in breast carcinoma cells. | Scientific Reports

Figure 3: LOXL2 depletion diminishes UPRE and ERSE promoter activities in breast carcinoma cells.

From: LOXL2 drives epithelial-mesenchymal transition via activation of IRE1-XBP1 signalling pathway

Figure 3

(a) Promoter assays were performed in control mouse Eo771 (pLKO) (left) and human MDA-MB-231 cells (shEGFP) (right) and in cells silenced for LOXL2 (shLOXL2). Error bars represent the s.e.m. (n = 3) (*p < 0.05, **p < 0.01, ***p < 0.001). (b) LOXL2 knockdown in Eo771 and MDA-MB-231 cells abrogates XBP1 splicing. Cells used in (a) were processed for RT-PCR analysis of spliced XBP1. GAPDH levels serve as loading control. Unspliced (XBP1u) and spliced (XBP1s) forms of XBP1 are indicated. (*) XBP1 hybrid band. One representative RT-PCR analysis of three independent experiments is shown.

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