Figure 12: ERK1/2 and NFATc1 signaling pathways interacted with each other in porcine skeletal muscle satellite cells. | Scientific Reports

Figure 12: ERK1/2 and NFATc1 signaling pathways interacted with each other in porcine skeletal muscle satellite cells.

From: Akirin2 regulates proliferation and differentiation of porcine skeletal muscle satellite cells via ERK1/2 and NFATc1 signaling pathways

Figure 12: ERK1/2 and NFATc1 signaling pathways interacted with each other in porcine skeletal muscle satellite cells.

(A,B) PD98059 or DMSO was added 1 h before the transfection of pcDNA3.1(+)-pAkirin2. After cell proliferation (A) treatment with Akirin2 for 24 h or differentiation (B) treatment with Akirin2 for 72 h, total cell lysates were subjected to SDS-PAGE and immunoblotted with anti-NFATc1 and GAPDH antibodies. (C,D) CsA or DMSO was added 1 h before the transfection of pcDNA3.1(+)-pAkirin2. After cell proliferation (C) treatment with Akirin2 for 24 h or differentiation (D) treatment with Akirin2 for 72 h, total cell lysates were subjected to SDS-PAGE and immunoblotted with anti-phosphorylation of ERK1/2 and ERK1/2 antibodies. Results were presented as mean ± SE (n = 3). Values with different letters are significantly different (P < 0.05).

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