Figure 4: Effect of Akirin2 overexpression and Akirin2 silencing on the proliferation of porcine skeletal muscle satellite cells. | Scientific Reports

Figure 4: Effect of Akirin2 overexpression and Akirin2 silencing on the proliferation of porcine skeletal muscle satellite cells.

From: Akirin2 regulates proliferation and differentiation of porcine skeletal muscle satellite cells via ERK1/2 and NFATc1 signaling pathways

Figure 4: Effect of Akirin2 overexpression and Akirin2 silencing on the proliferation of porcine skeletal muscle satellite cells.

Approximately 60% confluent porcine skeletal muscle satellite cells were transfected with 0.5 μg of pcDNA3.1(+)-pAkirin2 or 50 nM of Akirin2-siRNA and cultured in proliferation medium for 24 h. (A,C) Cell proliferation was evaluated by EdU proliferation assay. The percentage of EdU-positive cells was quantified. Results were presented as mean ± SE (n = 6). (B,D) Western blot analysis of extracts from porcine skeletal muscle satellite cells by using anti-phospho-Histone H3 antibody. Results were presented as means ± SE (n = 3). *P < 0.05 and ***P < 0.001 as compared with the control group.

Back to article page