Figure 5: Effect of Akirin2 overexpression and Akirin2 silencing on the differentiation of porcine skeletal muscle satellite cells. | Scientific Reports

Figure 5: Effect of Akirin2 overexpression and Akirin2 silencing on the differentiation of porcine skeletal muscle satellite cells.

From: Akirin2 regulates proliferation and differentiation of porcine skeletal muscle satellite cells via ERK1/2 and NFATc1 signaling pathways

Figure 5: Effect of Akirin2 overexpression and Akirin2 silencing on the differentiation of porcine skeletal muscle satellite cells.

(A) Overexpression of Akirin2 promoted porcine skeletal muscle satellite cells differentiation. Approximately 80% confluent porcine skeletal muscle satellite cells were transfected with 1 μg of pcDNA3.1(+)-pAkirin2 and cultured in differentiation medium for 72 h. Total cell lysates were subjected to SDS-PAGE and immunoblotted with anti-Akirin2, MHC and GAPDH antibodies. (B) Akirin2 silencing inhibited porcine skeletal muscle satellite cells differentiation. Approximately 80% confluent porcine skeletal muscle satellite cells were transfected with 50 nM of Akirin2-siRNA and cultured in differentiation medium for 72 h. Total cell lysates were subjected to SDS-PAGE and immunoblotted with anti-Akirin2, MHC and GAPDH antibodies. Results were presented as means ± SE (n = 3). ***P < 0.001 as compared with the control group.

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