Figure 6: Akirin2 activates ERK1/2 signaling pathway in proliferating porcine skeletal muscle satellite cells.

Approximately 60% confluent porcine skeletal muscle satellite cells were transfected with 0.5 μg of pcDNA3.1(+)-pAkirin2 and cultured in proliferation medium for 24 h. Total cell lysates were subjected to SDS-PAGE and immunoblotted with anti-phospho-ERK1/2 and ERK1/2 antibodies. Results were presented as mean ± SE (n = 3). ***P < 0.001 as compared with the control group.