Figure 7: Akirin2 promotes porcine skeletal muscle satellite cells proliferation through ERK1/2 signaling pathway. | Scientific Reports

Figure 7: Akirin2 promotes porcine skeletal muscle satellite cells proliferation through ERK1/2 signaling pathway.

From: Akirin2 regulates proliferation and differentiation of porcine skeletal muscle satellite cells via ERK1/2 and NFATc1 signaling pathways

Figure 7: Akirin2 promotes porcine skeletal muscle satellite cells proliferation through ERK1/2 signaling pathway.

(A) Porcine skeletal muscle satellite cells were cultured in proliferation medium. PD98059 (50 μM) or DMSO was added 1 h before transfection with 0.5 μg of pcDNA3.1(+)-pAkirin2 in approximately 60% confluent porcine skeletal muscle satellite cells. After 24 h, cell proliferation was evaluated by EdU proliferation assay. The percentage of EdU-positive cells was quantified. Results were presented as mean ± SE (n = 6). (B) Lysates from porcine skeletal muscle satellite cells treated as described in (A) were immunoblotted with anti-phospho-Histone H3 antibody. Equal loading was monitored with anti-GAPDH antibody. Results were presented as mean ± SE (n = 3). Values with different letters are significantly different (P < 0.05).

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