Figure 8: Akirin2 promotes porcine skeletal muscle satellite cells differentiation through ERK1/2 signaling pathway.

PD98059 or DMSO was added 1 h before transfection with 1 μg of pcDNA3.1(+)-pAkirin2 in approximately 80% confluent porcine skeletal muscle satellite cells. On day 3 of differentiation, total cell lysates were subjected to SDS-PAGE and immunoblotted with anti-MHC and GAPDH antibodies. Results were presented as mean ± SE (n = 3). Values with different letters are significantly different (P < 0.05).