Table 1 Linear mixed models of phenological variables in relation to sex and methylation at Clock poly-Q or 5′-UTR.

From: Migration phenology and breeding success are predicted by methylation of a photoperiodic gene in the barn swallow

 

poly-Q

5′-UTR

χ21

F

df

P

Coefficient (SE)

χ21

F

df

P

Coefficient (SE)

Departure date from wintering area

Year

0.00

    

0.00a

    

Area

0.00

    

0.00a

    

Sex

 

10.79

1,62

0.002

  

0.81a

1,61

0.371

 

Methylation

 

17.43

1,62

<0.001

  

0.13a

1,61

0.717

 

Sex × Methylation

 

10.20

1,62

0.002

  

1.54

1,60

0.219

 

Males

    

−0.29 (0.24)

    

0.57 (0.56)

Females

    

−2.15 (0.53)***

    

−0.58 (0.75)

Arrival date to breeding site

Year

9.30**

    

5.40a *

    

Area

3.00

    

3.70a

    

Sex

 

4.38

1,57

0.041

  

2.16a

1,57

0.147

 

Methylation

 

17.64

1,57

<0.001

  

0.60a

1,57

0.440

 

Sex × Methylation

 

3.74

1,57

0.058

  

0.00

1,56

0.997

 

Males

    

−0.59 (0.22)**

    

−0.31 (0.52)

Females

    

−1.61 (0.50)**

    

−0.31 (0.67)

Breeding date

Year

7.70**

    

7.70a **

    

Area

4.30*

    

4.30a *

    

Sex

 

3.67

1,77

0.059

  

6.39a

1,78

0.014

 

Methylation

 

4.97

1,77

0.029

  

2.65a

1,78

0.108

 

Sex × Methylation

 

4.60

1,77

0.035

  

1.45

1,77

0.232

 

Males

    

−0.02 (0.19)

    

−0.95 (0.47)

Females

    

−0.97 (0.40)*

    

0.14 (0.77)

  1. *0.05 > P > 0.01; **0.01 ≥ P ≥ 0.001; ***P < 0.001. aEstimated from a model excluding the interaction term. χ21: likelihood ratio test for the random effect of year or area. Methylation (percentage of 5-methyl-cytosine) is included as a covariate. Year and area are included as random factors; sex as a fixed-effect factor. Sample sizes for the analyses of poly-Q were as follows (males, females): departure date from wintering area: 49, 20; arrival date to breeding site: 45, 19; breeding date: 58, 26. The corresponding values for the analyses of 5′-UTR were: 48, 19; 45, 18; 59, 25; 61, 25.