Figure 6: Structural, Neighbor-joining tree and Expression profiles of members of the CPAP gene families (CPAP1 and CPAP3).
From: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome
(A) The structure of CPAP cuticular proteins. Red indicates the predicted signal peptide, green indicates the chitin-binding peritrophin-A domain (ChBD2). (B) A phylogenetic tree was constructed with the neighbor-joining method of MEGA 5 using the pairwise deletion of indels. Bootstrap support was based on 1,000 resampled data sets. The GenBank accession numbers are listed in Table S1. (C) Expression of CPAP1 and CPAP3 in different tissues on day 6 of 5th instar nymphs as detected by RT-qPCR. Different tissues are listed: Integument, foregut, midgut, gastric caeca, hindgut, Malpighian tubules, fat body; (D) Expression of CPAP1 and CPAP3 in the integuments of 5th instar nymphs at different days as detected by RT-qPCR. N5D1, N5D3, N5D5, N5D7: Day 1, Day 3, Day 5, day 7 of 5th instar nymphs. β-actin was used as the reference control. All data are reported as means ± SE of three independent biological replications. Heat map showing relative expression level during different tissues and stages of nine CPAP cuticular protein genes. The colors in map display the relative values of all tiles within the given 7 different tissues or 4 developmental stages. Blue indicates the lowest expression, green indicates the intermediate expression, and red indicates the highest expression. The color scale bar is shown on the right of the figure.
