Figure 7: Neighbor-joining tree and Expression profiles of members of the Tweedle gene families.
From: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome
(A) A phylogenetic tree was constructed with the neighbor-joining method of MEGA 5 using the pairwise deletion of indels. Bootstrap support is based on 1000 resembled data sets. GenBank accession numbers are listed in Table S1. (B) Expression of Tweedle genes in different tissues on day 6 of 5th instar nymphs as detected by RT-PCR (28 cycles). β-actin was used as the reference control (24 cycles). Different tissues are listed: Integument, head, leg, goad, gut, Malpighian tubules, fat body, wing pad, pronotum; The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 5. (C) Expression of Tweedle genes in the pronotum of 5th instar nymphs at different days as detected by RT-qPCR. N5D1, N5D3, N5D5, N5D7: Day 1, Day 3, Day 5, day 7 of 5th instar nymphs. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.
