Figure 8: Expression profiles of members of the CPF/CPFL gene families as determined by RT-PCR and RT-qPCR.
From: Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome
(A) Expression of CPF/CPFL gene-annotated nymph cuticle protein genes in different tissues on day 6 of 5th instar nymphs as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, head, leg, pronotum, goad, gut, Malpighian tubules, fat body, wing pad; (B) Expression of CPF/CPFL gene-annotated adult cuticle protein genes in different tissues on day 2 of adults, as detected by RT-PCR (28 cycles). Different tissues are listed: Integument, leg, muscle, tentacle, goad, gut, Malpighian tubules, fat body, wing. β-actin was used as the reference control (24 cycles). The image were obtained by using the gel imaging analysis system (Bio-Rad, USA). The full-length gels are presented in Supplementary Fig. 6. (C) Expression of CPF/CPFL genes in the pronotum after molt from 4th instar nymphs to 5th instar nymphs at 0 h, 36 h, 72 h and 96 h, as detected by RT-qPCR. β-actin was used as the reference control. Data are reported as means ± SE of three independent biological replications.
