Figure 3: Mitochondria with onion-like swirling cores accumulated in RM1L flies during aging.

(A) Representative thin-section EM images of mitochondria with swirling membranes and surrounded swollen spaces. (B) The population of swirling mitochondria in WT, RWT, RM1L at week 1, 4, and 7. (C) Representative tomographic slices and the corresponding segmentation (right panels) showing a cross-sectional view (top) and a longitudinal-section view (bottom) of a swirling core. Volume segmentation was displayed in arbitrary color rendering to highlight the swirling core. Scale bar: 200 nm. Scale bar: 1 μm (A, top panel), 0.2 μm (A, bottom panel), 200 nm (C).