Figure 4: Mitochondria with swirling membranes retained partial function in sub-sections containing lamellar cristae. | Scientific Reports

Figure 4: Mitochondria with swirling membranes retained partial function in sub-sections containing lamellar cristae.

From: Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging

Figure 4

(A) Immuno-EM analysis of ATP synthase showing an absence in swirling membranes and presence in lamellar membranes (arrow: immune-gold). (B) COX activity assay showing weak staining in swirling membranes and strong staining in lamellar membranes. Immuno-EM analysis of PDHA1 (C) and SOD2 (D) showing preferential distribution in regions containing lamellar cristae compared to swirling membranes. Of note, the labeling was mostly absent from surrounding muscle tissue that showed relative good labeling specificity. Immuno-EM labeling of dsDNA (E) and DNA-RNA hybrids (F) showing the presence of both species around swirling membranes. Left panels of (E and F) showed regions of nuclei as a demonstration of labeling specificity. Scale bar: 0.5 μm.

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