Table 1 Sedimentation velocity on 0.25 mg/ml VanS in HGN buffer (10 mM, HEPES, 100 mM NaCl + 20% glycerol).

From: Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin

 

sT,b (S)

s20,w (S)

% w/w

Main component (monomer)

0.9 ± 0.1

2.3 ± 0.1

96%

2nd peak

4.3 ± 0.2

10.9 ± 0.5

4%

  1. Experiments conducted at 20.0 °C at a rotor speed of 40,000 rpm (check). 1S = 10−13 sec. sT,b: the value measured in the temperature and buffer (solvent), not normalized to standard conditions. s20,w: after correction/normalization to standard solvent conditions – the density and viscosity of water at 20.0 °C.
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