Figure 3: ATAD3A and HSPD1 contribute to virus multiplication.

(A) BmNPV multiplication was measured on ATAD3A and HSPD1 overexpression cells by flow cytometric analysis and ATAD3A, HSPD1 and Mock were infected with BmNPV at different time-points. (B) Statistical analysis of the EGFP+ positive cells in the indicated stable cell lines after BmNPV infected at different time-points. **Represents statistically significant differences at the level of P < 0.01. (C) Western blotting analysis of VP39 protein synthesis after overexpression with ATAD3A, HSPD1 and Mock at different times in BmNPV-infected BmN-SWU1 cells. (D) BmN-SWU1 cells were treated with indicated plasmid and infected with BmNPV at MOI of 10. At 0, 3, 6, 12, 24, 48 and 72 h.p.i., total intracellular DNA was extracted from infected cells. BmNPV copy numbers were determined by qPCR. Each data point was determined from the mean of three independent replicates. NS, not significant. **Represents statistically significant differences at the level of P < 0.01.