Table 1 Time-dependent changes in CD signal in absence or presence of 10- or 50-fold molar excess of SAM.

From: One amino acid makes a difference–Characterization of a new TPMT allele and the influence of SAM on TPMT stability

Residual CD signal remaining after 8 hours measurements a (%)

Protein variant

−SAM

+SAM10-fold

+SAM50-fold

TPMTwt

85

97***

105***

TPMT p.Y240C

75

80

90***

TPMT p. Y240S

77

77

75

  1. CD signal monitored at 222 nm. ***Significant level of one-way ANOVA: p ≤ 0.001.
  2. aThe percentage of CD signal remaining after 8 hours of measurements at 37 °C.
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