Table 2 PCR and sequencing primers.

From: One amino acid makes a difference–Characterization of a new TPMT allele and the influence of SAM on TPMT stability

Target Exon

Forward primer (5′ to 3′)

Reverse primer (5′ to 3′)

M13 tailed intronic primers a

 V

TGTAAAACGACGGCCAGTCTGCATGTTCTTTGAAACCCTATG

CAGGAAACAGCTATGACCCTGCGTGCTAAATAGGAACCATC

 VII

TGTAAAACGACGGCCAGTGGGACGCTGCTCATCTTCT

CAGGAAACAGCTATGACCGCCTTACACCCAGGTCTCTG

 X

TGTAAAACGACGGCCAGTAAGTGTTGGGATTACAGGTG

CAGGAAACAGCTATGACCTCCTCAAAAACATGTCAGTGTG

Sequencing exon 2 to 10

 II

AGCCTGGGGGATAGAGAGAG

GCAAACATTGCATAAAAGCAT

 III

AGGTTTTCATTTAGTTCATCAAT

TTTTTGATAGAACATTTCTCTATTGT

 IV

TACCACTGACTGGGTGTGTGTCTGA

CTCAATCCAGAAAGACTTCATACCTGTT

 V

CCCTCTATTTAGTCATTTGAAAAC

ACTTTTGTGGGGATATGGAT

 VI

GCCCTCTTTCCTTGACTATT

CACAGGAGGAAGAGAGTGAG

 VII

TGTTGAAGTACCAGCATGCAC

AAAATTACTTACCATTTGCGATCA

 VIII

CGAAAGTAACTTCTGGCTTC

GGCAACTGGTAAAAGAAAAA

 IX

ATGCCACATCATCACCTATT

ACAGCATAAGTCCACAAACTT

 X

CACCCAGCCAATTTTGAGTA

CACCCAGCCAATTTTGAGTA

Pyrosequencing

 X

Biotin-CCCAGCCAATTTTGAGTATT

CAATTCCTCAAAACATGTCA

 

Sequencing primer

 
 

TTTACTTTTCTGTAAGTAGA

 
  1. aPCR primers with M13 tails in italic.
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