Figure 7

Photomicrographs for LFB/PAS (x200) stained coronal sections of the corpus callosum from (A) normal control group (B) free rhMBP treated control group (C) rhMBP NPs treated control group displaying blue stained tracts in myelinated corpus callosum. (zero score for demyelination) (D) EAE group indicating complete demyelination with wide tissue vacuolation and loss of the blue stained fibers. (demyelination score = 3). Arrows point to myelin fragments in the corpus callosum. (E) free rhMBP pretreated EAE group showing mild preservation of myelin sheath with focal tissue vacuolation and moderate loss of the blue stained fibers. (demyelination score = 2). Arrows point to myelin fragments in the corpus callosum. (F) rhMBP NPs pretreated EAE group indicating better preservation of myelin sheath with minimal tissue vacuolation and mild loss of the blue stained fibers. (demyelination score = 1). Arrow point to myelin fragments in the corpus callosum. The brains were processed 14 days post induction.