Figure 2: Developmental and reproduction-related roles of PAPLA1.

(a) PAPLA1 deficiency reduces hatchability (egg to 1^st larval instar survival). Fischer’s exact test: P < 0.001. (b) PAPLA1 deficiency reduces viability (survival from the 1^st larval instar to adulthood). Larvae that did not give rise to adult flies are denoted as non-viable. Fischer’s exact test: P < 0.001. (c) PAPLA1 deficiency reduces developmental speed. Shown are mean % of pupariated flies at the given time point ± SEM. Mann Whitney test to compare median developmental times: P < 0.001 for both PAPLA1 mutants. (d) PAPLA1 deficient flies develop into pupae with standard volume. Two-tailed Student’s t–test: P > 0.05. (e) PAPLA1 deficiency does not affect body size of adults (measured as wing area). Two-tailed Student’s t–test: P > 0.05 for both assays. (f) PAPLA1 deficient flies have lowered egg production. Two-tailed Student’s t–test: P < 0.001.