Figure 2
The effect of IL-1β on glycogen synthase kinase-3β (GSK-3β) activity in differentiated hippocampal neural stem/progenitor cells (NSPCs). (a) Representative photomicrographs of cells immunocytochemically stained for doublecortin (DCX) (red), βIII-tubulin (red) or glial fibrillary acidic protein (GFAP) (red) with GSK-3β (green) in untreated control and interleukin-1β (IL-1β)-treated cultures after 7 DIV under differentiation conditions. Cells were counterstained with DAPI (blue) to visualize the nuclei. Scale bar=50 μm. (c and e) Immunoblot analysis of β-catenin, p-GSK-3β (serine 9), total GSK-3β and β-actin protein levels in total cell lysates from cells cultured in the presence or absence of IL-1β. Protein bands of 92, 42, 46 and 46 kDa indicate β-catenin, β−actin (c) p-GSK-3β (serine 9) and total GSK-3β (e) expression, respectively. (b and d) Mean densitometry analysis of β-catenin equalized to β-actin (b), and p-GSK-3β equalized to total GSK-3β (d) from three independent experiments. Data are expressed as mean±s.e.m. *P<0.05, ***P<0.001 vs untreated control (Students t-test).
